PICO Amplification Core Kit

The PICO Amplification Core (AMC) Kit enables the highly sensitive detection and quantification of proteins, protein interactions, and post-translational modifications. The PICO AMC workflow includes the chemical crosslinking and lysis of the sample, followed by the incubation of lysates with the labeled antibodies. After the binding reaction the sample is highly diluted and loaded into a QIAcuity Nanoplate 26k 24-well. During the dPCR reaction in QIAGEN's QIAcuity dPCR System, the PICO labels are amplified and fluorescent signal is generated and recorded. After the dPCR run, the raw data is uploaded and analyzed with our online AMULATOR software.

The PICO AMC Kit is designed to perform 120 dPCR reactions corresponding to five full 24-well dPCR plates or five PICO experiments.

The following PICO products are required additionally in the PICO AMC workflow:

• PICO BL Probe
• PICO P8 Probe
• PICO N6 Probe
• PICO O7 Probe

The following QIAGEN consumables are required additionally in the PICO AMC workflow:

• QIAcuity Nanoplate 26k 24-Well
• QIAcuity Probe PCR Kit